《植物生理学报》 2010, 46(6): 571-574

6-BA 对红掌组织培养中红叶变异的影响

丁爱萍*, 史正军

深圳市园林科学研究所, 广东深圳 518003

通信作者：丁爱萍；E-mail: dingapsz@sohu.com；Tel: 0755-25400643

摘　要：

以红掌盆栽品种 ‘Avo-Gloria’ 为试材, 以 MS+0.2 mg•L-1 2,4-D 为基本培养基, 分别在添加 1~10 mg•L-1 6-BA 的 10 种脱 分化培养基上, 诱导其叶柄外植体产生愈伤组织; 再以MS+2 mg•L-1 6-BA+0.2 mg•L-1 NAA为分化培养基诱导分化不定芽; 以 MS+0.2 mg•L-1 NAA 为生根培养基, 从不定芽获得再生植株。结果显示: (1)在MS+0.2 mg•L-1 2,4-D+8~10 mg•L-1 6-BA 的3 种 脱分化培养基上可产生 9%~10% 的绿色、质地较硬的愈伤组织; (2)愈伤组织在 MS+2 mg•L-1 6-BA+0.2 mg•L-1 NAA 的分化 培养基上, 经6~8次继代培养, 可获得3%~7%的不定芽, 并可生根长成再生植株; (3)再生植株定植3个月后, 有3%~7%植株 出现红叶变异, 此红叶可终生表现为红色。

关键词：红掌; 组织培养; 红叶变异; 6-BA

收稿：2010-02-02   修定：2010-04-12

资助：深圳市重点科技计划项目(2003-2-033)。

Influence of 6-BA on Leaf Mutation of Tissue-Cultured Anthurium andraeanum Lind.

DING Ai-Ping*, SHI Zheng-Jun

Shenzhen Institute of Landscape Science, Shenzhen, Guangdong 518003, China

Corresponding author: DING Ai-Ping; E-mail: dingapsz@sohu.com; Tel: 0755-25400643

Abstract:

Leafstalk explants of Anthurium andraeanum pot variety ‘Avo-Gloria’ were induced to form callus in 10 different de-differentiation mediums. The de-differentiation mediums had MS+0.2 mg·L^-1 2,4-D as essential medium with different 6-BA additions, which were ranging from 1 mg·L^-1 6-BA to 10 mg·L^-1 6-BA, respectively. Differentiation of adventitious buds was induced in medium of MS+2 mg·L^-1 6-BA+0.2 mg·L^-1 NAA, and MS+0.2 mg·L^-1 NAA was used as rooting medium to regenerate plants from the adventitious buds. The results showed that the de-differentiation medium with MS+0.2 mg·L^-1 2,4-D+8–10 mg·L^-1 6-BA could generate 9%–10% green and relatively hard-textured callus. Subcultured 6–8 times on differentiation mediums with MS+2 mg·L^-1 6-BA+0.2 mg·L^-1 NAA could get 3%–7% adventitious buds, which could become regenerated plants. After transplanting the regenerated plants for 3 months, 3%–7% of the plants have leaf mutation, and the mutated leaves can be red whole life.

Key words: Anthurium andraeanum; tissue culture; leaf mutation; 6-BA